Part:BBa_K4047034:Experience

This part was found to be functional in the experimental work of the MiamiU_OH 2021 team, with successful integration into the neutral site confirmed through PCR. The integrated sequence was confirmed to be the same one shown here through genetic sequencing, confirming no mutations occurred. This gene was integrating using the plasmids BBa_K4047036 and BBa_K4047038. The focus here is presented with data from BBa_K4047036 due to only transaldolase being expressed in those transformants.

Although proteomics had not yet been completed by the end of the competition timeframe, transformants did display different growth than wild type cells, indicating a successful shift in protein activity, as shown in the attached figure. Considering this gene is sourced from the chassis genome itself and maintained sequence faithfulness to the original source when integrated, we can safely conclude that the expressed proteins were functional transaldolase. We can also conclude that the differential protein activity was due to transaldolase overexpression, as this was the only change induced.

Growth of Synechococcus elongatus cells when induced to overexpress transaldolase or fructose-1,6-bisphosphatase compared to wild type cells, as recorded in the MiamiU_OH 2021 project.

Applications of BBa_K4047034

This coding sequence for transaldolase can be used to overexposes the corresponding enzyme, allowing manipulation of central metabolism in Synechococcus species.

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